IMMUNOMODULATORY ACTIVITY OF NUTRACEUTICAL FORMULATION AND ITS POTENTIATION BY SELF-FORTIFICATION AND COW URINE DISTILLATE FORTIFICATION METHODS

摘要

ABSTRACT Objective: This present study was designed to prepare, evaluate immunomodulatory activity of nutraceutical formulation and to study the effect of self fortification and cow urine distillate fortification methods on immunomodulatory potential of nutraceutical formulation. Methods: Three types of nutraceutical formulations NF, SFNF and SFNECUD were prepared using dried powders of amla, apple, garlic, onion, wheat grass, papaya, turmeric and cow urine distillate by different methods. The immunomodulatory activity of nutraceutical formulations at a dose of 500 mg/kg was assessed by various immune function parameters like cell mediated immunity (Neutrophil adhesion, DTH response and cyclophosphamide induced neutropenia), humoral immunity (Serum immunoglobulins level and haemoagglutination antibody titer), and Phagocytic activity (Carbon clearance and PMN cell activity). Results: Oral administration of NF, SFNF and SFNFCUD showed significant (p<0.01) increase in adhesion of neutrophils, potentiation of the DTH reaction and attenuation of cyclophosphamide-induced neutropenia. A significant increase in serum immunoglobulin levels and production of circulating antibody titer in response to sheep red blood cells (SRBCs) was also observed. In addition, an increase in phagocytic index in carbon clearance assay and increase in the phagocytic activity of PMN cells was observed. Conclusion: From the above results, it can be concluded that all three types of formulations showed significant immunostimulant activity. SFNF and SFNFCUD showed better immunomodulatory activity than NF suggesting the potentiation of immunomodulatory potential of NF activity by fortification methods. Key words: Nutraceutical formulation, Fortification, Cell mediated immunity, Humoral immunity and Phagocytic activity.