PHYTOCHEMICAL ANALYSIS OF N-HEXANE LEAF EXTRACT OF ALPINIA PURPURATA (VIEILL.) K. SCHUM USING UV-VIS, FTIR AND GC-MS

摘要

Objective: The present study was carried out to characterize bioactive constituents present in n-hexane leaf extract of Alpinia purpurata (Vieill.) K. Schum. Methods: Phytochemical screening of the leaf extract of Alpinia purpurata revealed the presence of some bio-active components. The crude extracts were scanned in the wavelength ranging from 200-800 nm by using Ultraviolet-Visible (UV-Vis) spectrophotometers. Fourier transform infrared spectrophotometer (FTIR) was used to determine the functional groups in the plant. Gas chromatography-mass spectrometry (GC-MS) analysis was also performed to find major phytoconstituents present. Results: The phytochemical tests showed the presence of alkaloids, terpenoids, flavonoids, steroids, cardioglycosides, oils and fats, tannins and carbohydrates in n-hexane leaf extract of A. purpurata. In UV-Vis analysis there were sharp peaks from 200-700 nm. In FTIR analysis, the plant showed the presence of ester carbonyl and unsaturated carbonyl groups in 1708 and 1691 cmˉ1 respectively. There were strong absorption bands at 2927 and 1452 cmˉ1 due to CH and CH 2 groups. The GC-MS analysis revealed the presence of different phytochemical compounds . This is the first time the presence of 4-Morpholinomethyl-7-methoxycoumarin 1.42%, Methanesulfonate of (3R,4S)-3-Propargyloxy-4-[(R)-1-hydroxy-3-phenyl-3-butenyl]-1-(p-methoxyphenyl)-2-azetidinone 2.28%, 5-Butyl-3-Methyl-1,2,3, 8a-Tetrahydroindolizine 6.48%, Phenol, 4-(3,7-dimethyl-3-ethenylocta-1,6-dienyl)-6.99%, 1-Naphthalenepropanol, à-ethenyldeca hydro-à,5,5,8a-tetramethyl-2-methylene-[1S-[1à (R*),4aá, 8aà]]-9.29%, Methenolone 10.93%, and Nonanamide, 5-hydroxy-5-methyl-2-(2-methylpropyl)-N-benzyl-25.80% were reported on the leaf extract of Alpinia purpurata. Conclusion : From the results, it is evident that A. purpurata has various phytoconstituents and functional groups. The intensive study of the resultant active constituents will lead to the discovery of a novel botanical-drug.