QUALITY CONTROL OF CINNAMIC ACID IN TRADITIONAL MEDICINAL PLANTS IN JORDAN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

Objective: The objective of the study: is the analysis of cinnamic acid (CA) from commercial herbal drugs, and from dried plant material used in traditional medicine in Jordan market qualitatively and quantitatively by using (HPLC) high performance liquid chromatography. Methods: The chromatographic conditions comprised of a reversed-phase RP-8 column with a mobile phase consisting of a mixture of Acetonitrile: Phosphate buffer solution (35:65 v/v) with flow rate 1 ml/min. The detection was carried out at 269 nm, with retention time of 17.3 min. The standard curve was linear over the range of (20-400) μg/ml with correlation coefficient of the linear calibration graphs for the analytics exceeded (0.9987). Results: A Simple, sensitive and specific HPLC method was developed and validated for a qualitative and quantitative analysis of cinnamic acid from commercial herbal drugs, and from dried plant material used in traditional medicine in Jordan market. Conclusion: The highest concentration of cinnamic acid was found in Cinnamon verum(Lauraceous) (4330 μg/g) while the other plants contain insignificant amount of cinnamic acid as it is a key intermediate precursor for many plant metabolites. The cinnamic acid and derivate were successfully separated using pH 2.5.The developed method was successfully applied to estimate the amount of cinnamic acid in the main traditional medicinal plants in Jordan market