DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF LAFUTIDINE AND RABEPRAZOLE SODIUM IN COMBINED DOSAGE FORM

摘要

Objective: A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Lafutidineand RabeprazoleSodium in combined dosage form.Method:A Thermo Hypersil, C18 column, 250 mm × 4.6 mm, 5μm in Isocratic mode with mobile phase containing Acetonitrile:0.02M Pottasiumdihydrogenortho phosphate pH 7.2 (50:50 v/v) was used.The flow rate was 1.5ml/min and effluents were monitored at 215 nm. The retention time of Rabeprazole Sodium and Lafutidinewas found to be 2.99 min and 8.13 min respectively.Result:The different analytical parameters such as accuracy, linearity, precision, robustness, limit of detection(LOD), limit of quantification (LOQ) were determined according to the International Conference on Harmonization (ICH ) Q2R1 guidelines. The detector response was linear in the range of 40-120 μg/ml, 80-240 μg/ml for Lafutidineand RabeprazoleSodium respectively.Conclusion: The Proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Pharmaceutical dosage form