RP-HPLC Method for the Simultaneous Estimation of Lamivudine and Abacavir Sulphate in Tablet Dosage Form

摘要

A rapid high performance liquid chromatographic methods has been developed and validated for the estimation of Lamivudine and Abacavir simultaneously in combined dosage form, separation was performed on a 5μm C18 column having dimensions (150X4.6mmid) in isocratic mode, with mobile phase containing a mixture of methanol: water (70:30, v/v/) was used. The Mobile phase was pumped at a flow rate of 1.4 ml/min and eluents were monitored at 275nm. The selected chromatographic conditions were found to separate Lamivudine (Rt= 2.549 min) and Abacavir (Rt= 3.499 min) having a resolution of 4.13 min. The method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantification. Linearity of Lamivudine and Abacavir were found in the range of 2 -12 μg/ml. The percentage recoveries for Lamivudine and Abacavir ranged from 99.18 ±101.00% and 99.83±100.83% respectively. The limit of detection was found to be 0.0268 and 0.0049 for Lam and Aba respectively. Limit of quantification was found to be 0.0184 and 0.0150 for Lam and Aba respectively. The proposed method can be used for the estimation of these drugs in combined dosage form.