Study On The Effects Of Heavy Metals? Pollution On The Activity Of 7-Ethoxyresorufin-O-Diethylase (EROD) In Two Freshwater Fish Species Of Indonesia

摘要

The study aimed to determine the effect of heavy metal pollution on 7-ethoxyresorufin-O-diethylase (EROD) enzyme activity in carp (Cyprinus carpio) and tilapia (Oreochromis niloticus). The samples were taken by purposive random sampling technique. The treatment site was the downstream part of the Kaligarang River, Semarang, Indonesia, which contaminated byheavy metals (concentration of Cd 0.007 ppm, Pb 0.010 ppm, and Hg 0.0006 ppm). A clean reference site in Nyatnyono Village, Ungaran was used as the control site with the heavy metal content of 0 ppm. The EROD activities in carp and tilapia were significantly decreased after Cd, Pb, and Hg exposure. The results showed that the average value of liver EROD activity in carp fish control group amounted to 1.77 ' 0.23'mol/min/mg protein and the treatment group was 0.49 ' 0.24'mol/min/mg protein. Whereas in control group of tilapia fish, EROD activity was equal to 2.08 ' 0.47'mol/min/mg protein and the treatment group was 0.49 ' 0.40'mol/min/mg protein. The results demonstrated that metallothionein was a specific heavy metals biomarker, whereas EROD activity ishighly sensitive to extremely low concentrations of theselected pollutants.In conclusion, the use of multiple biomarkers is recommended to monitor the heavy metal pollutants in the river environment.