Low Cost Production Of Biosurfactant From Different Substrates And Their Comparative Study With Commercially Available Chemical Surfactant.

摘要

Abstract: Biosurfactants are amphiphilic compounds produced by various bacteria and fungi which reduce surface and interfacial tension. In this work, the biosurfactant produced by Bacillus subtilis strain isolated from soil samples was characterized and its properties compared with commercially available chemical surfactants. Bacillus subtiliswas used for the production of biosurfactant and its activity was tested against crude vegetable oil. The crude biosurfactant was produced using four different substrates and its emulsification activity was compared against sodium dodecyl sulphate (SDS). The results showed that the isolated bio surfactant from coconut waste showed the highest emulsification activity even more than sodium dodecyl sulphate (SDS) which is a commercial chemical surfactant. Furthermore its antimicrobial activity was checked against Staphylococcus aureus, Escherichia coli, Pseudomonas and Salmonella typhimurium. The study concludes that coconut and soyabean waste are the most ideal substrate for biosurfactant biosynthesis, which may have potential industrial applications.