Determination of Phytochemicals and Minimum Inhibitory Concentrations of Leaf Extracts of Camellia sinensis and Murraya koenigii on MRSA and MSSA strains and virulence factors of the strains

摘要

Virulence traits (coagulase, hemolysin, protease, catalase, lipase, slime layer, biofilm and hydrophobicity of 33 MSSA and 11 MRSA strains were studied. All the MRSA and MSSA isolates exhibited lipase, coagulase and catalase activity. The proportion of MRSA isolates producing protease, hemolysin, biofilm and slime layer was higher as compared to MSSA. MRSA appeared more hydrophobic in salt aggregation test and better biofilm producers. The sugar supplemented media (trypticase soya broth and brain heart infusion broth) were better for biofilm production. The susceptibility of all the isolates to aqueous and alcoholic leaf extracts of C. sinensis and M. koenigii was determined in the order: (aqueous < ethanolic < methanolic). MIC values of both the alcoholic extracts of M. koenigii (curry leaves) were lower as compared to that of C. sinensis. These values were higher for MRSA. MIC50 values of the ethanolic (3.75 x 102 mg/ml) and methanolic extract (6.25 x 102 mg/ml) of M. koenigii were lower as compared to the alcoholic extracts of C. sinensis for MRSA isolates. In case of MSSA isolates, there was no difference in the MIC50 values of ethanolic extracts i.e 3.75 x 102 mg/ml of both the plants but in case of methanolic extracts, the MIC 50 value of C. sinensis was lesser as compared to that of M. koenigii. The extracts of both the plants revealed different phytochemicals.