A Study of Socio-Demographic and Behavioral Risk Factor in Patients with Cardiovascular Disease Visiting Rural Hospital in South India

摘要

Production of antimicrobial substances is often regarded a priori as an important trait in the context of bacterial fitness but also in terms of probiotic efficacy. Several probiotic bacteria produce a variety of antimicrobial compounds viz. short chain fatty acids, Hydrogen peroxide, Nitric oxide, Bacteriocins. Bacteriocins are peptide or protein complexes showing antibacterial activity against closely related species. These antimicrobial agents are gaining more and more attention as an alternative therapeutics not only in pharmaceutical but also as a preservative in food industries. In present study, bacteriocin production was carried out by Lactobacillus rennanquilfy WHL 3 isolated from whey and identified by 16s rRNA sequence analysis. This newly isolated strain showed antibacterial activity against several Gram positive and Gram negative bacteria. To obtain maximum bacteriocin yield, medium was optimized with various fermentation conditions like carbon and nitrogen source, Growth factors, pH and incubation temperature. An isolate WHL 3 showed the maximum bacteriocin production in modified MRS medium containing 1% Glucose, 1% Peptone, and 0.8% Yeast extract. Maximum bacteriocin production was observed at 40°C temperature at pH 6.0. Lactobacillus rennanquilfy WHL 3 is capable of producing bacteriocin at wide range of temperatures and hence it is an ideal strain for bacteriocin production for food and pharmaceutical industries.