Marantodes pumilum Leaves Promote Repair of Osteoporotic fracture In Postmenopausal Sprague-Dawley Rats

摘要

Background and Objective: Marantodes pumilum var alata ( MPva ), a herb product used in managing female reproductive health problems and postmenopausal symptoms, is known to protect the bone against osteoporosis. Despite convincing evidence of its anti-osteoporotic properties, its role on osteoporotic fracture is yet to be investigated. In this study, the effects of aqueous leaf extract of MPva on fracture repair were studied in ovariectomized rats. Materials and Methods: Thirty female Sprague-Dawley rats grouped (n = 6) into Sham-operated (Sham), Ovariectomized control (OVXC), Estrogen treatment (ERT) and leaf treatments (MPv20 and MPv100) groups were ovariectomized (except the Sham). Eight weeks post-ovariectomy, the right tibiae of rats were fractured and fixed with titanium plates. The ERT group received 64.5 μg kg^?1 per day p.o. estrogen (Premarin?) while the MPv20 and MPv100 groups received 20 mg and 100 mg kg^?1 per day p.o. doses of MPva leaf extract, respectively. After 8 weeks treatment, rats were humanely euthanized and their tibiae were excised for investigation of callus bone morphometry, mineralization and biomechanical strength. Results: Callus bone volume (BV_callus) and volume fraction (BV/TV_callus) were found to be higher (pConclusion: Similar to estrogen treatment and healthy control, treatment with aqueous leaves extract of Mpva promotes fracture healing in postmenopausal osteoporotic rats by enhancing the volume of bone callus formed at fracture site and its subsequent remodeling. In view of its safety profile, Mpva leaves could be a suitable complementary medicine in the management of osteoporotic fracture in postmenopausal condition.