Protective Role of Aerial Parts of Silene villosa Alcoholic Extract Against CCl₄-Induced Cardiac and Renal Toxicity in Rats

摘要

Background and Objective: Silene villosa (Family-Caryophyllaceae) is used traditionally in different part of Asian continents for the treatment of various illnesses. Recently, S. villosa has been tested and confirmed for anti-inflammatory and antioxidant activities. Therefore, the present study was carried out to evaluate cardiac and renal protective effects of S. villosa extract (SVE) against CCl₄-induced toxicity. Materials and Methods: The herbs of S. villosa were collected, authenticated, coarsely powdered and extracted in Methanol using percolation method. Wistar albino rats (≈200 g) of either sex were randomly divided into five groups. Group I (normal control) and Group II (toxic control) received normal saline (1 mL/100 g, p.o.) for 7 days. Groups III (positive control), IV and V (Test groups) were received Silymarin (10 mg kg^?1, p.o.), SVE at doses of 250 and 500 mg kg^?1, p.o., respectively for 7 days. On the 8th day, except normal group (Group I), all Groups were administered with CCl₄ (1.0 mL kg^?1, i.p). After 24 h of CCl₄ administration, the serum cardiac function parameters such as (LDH, CK and Albumin) and the serum renal function parameters such as creatinine, urea and uric acid , electrolytes and tissue total proteins were measured as a marker of cardiac and renal toxicity. The cardiac and renal tissue parameters such MDA and NP-SH were measured for the in vivo antioxidant activities. Further, the biochemical studies were followed by histopathological studies of heart and kidney tissues. Results: The treatment of CCl₄ in rats, significantly altered the cardiac and kidney function test limitation. Administration of SVE at two different doses of 250 and 500 mg kg^?1 with CCl₄ showed a significant (p4 intoxication by repairing the cardiac and kidney function abnormalities. The protective ability of SVE was further confirmed by the histological study of cardiac and renal tissues. Conclusion: The results led us to the conclusion that SVE ameliorate the cardiac and renal dysfunction.