Detection of Nonribosomal Peptide Synthetase (NRPS) Genes on Bacterial Endophytes fromVetiveria zizanioides L. and Ageratum conyzoides L.”

摘要

Study to analyze nonribosomal peptide synthetase (NRPS) genes diversity on bacterial endophytes isolates from roots of Vetiveria zizanioides and Ageratum conyzoides has been conducted. The aim of the research is to study the presence and diversity of NRPS genes on nine bacterial endophytes from root of medicinal plant. The bacterial DNA chromosomes has been isolated and amplified by Polymerase Chain Reaction (PCR) methods using primer which targeting adenilation domain of NRPS genes. The amplified segments were sequenced and analyzed in silico with bioinformatics methods. The 700 bp length DNA segment have been amplified on seven bacterial endophytes isolates of plant root. Bioinformatics analysis of the DNA sequence showed that all of the amplicon were coding NRPS enzymes that contain conservative domains of Adenylate Forming Domain (AFD) superfamily. Sequence motif that determine NRPS substrates detected from three sequences of bacterial endophytes. Phylogenetic studies indicates that NRPS genes from V. zizanioides endophytes were grouping with NRPS from Gammaproteobacteria group, otherwise NRPS genes from A. conyzoides were grouping with NRPS genes from Firmicutes Group. Study indicates that NRPS genes of bacterial endophytes from each host plants evolved separately.