IN VITRO EFFECT OF STERILIZING AGENTS ON CONTAMINATION AND REGENERABILITY OF JATROPHA CURCAS SEED: A BIOFUEL PLANT

摘要

The seeds of J.curcas were categorized into three groups on the basis of their size and naked embryo weight viz. small (1.0-1.75g), medium (2.0-2.75g), large (above3.0g).They were further sub divided into four types i.e. intact seed (with seed coat), halved seed, naked embryo (without seed coat) and halved naked embryo. The naked embryos of small, medium and large seeds expressed highest contamination (22.8%, 20.5% and 12.8%) when sterilized with HgCl2 (0.1%, 12 minutes). Contamination was lowest (11.4%, 7.5%, and 2.7 %) when treated with streptocycline (0.1%, 8minutes) + HgCl2 (0.1%, 21 minutes). The small, medium and large category seeds of both the types (intact seeds and naked embryo) exhibited maximum viability (4.9%, 5.6%, 6.5%) and (25.9%, 49.7%, 95.7%) respectively when treated with streptocycline (0.1%,8 minutes)+HgCl2(0.1%, 12 minutes). The naked embryo of large sized seeds sterilized with streptocycline (0.1%, 8 minutes) + HgCl2 (0.1%, 12minutes) exhibited maximum viability and lower contamination.