Variation of Antioxidant Trace Elements (Zinc and Copper) Status for Overweight Patients in Eastern of Algeria

摘要

Ocimum sanctum (Tulsi) is known to be an important medicinal plant from ancient period in India. Ursolic acid (UA), Oleanolic acid (OA) and Betulinic acid (BA), the derivatives of triterpenoid saponins, acts as a bioactive compounds and have been isolated from many medicinal plants and reported to possess a potent anti-bacterial activity against the pathogenic bacteria. The present study was aimed to undergo the phytochemical screening and to investigate the antibacterial efficacy of the leaf extract of Ocimum sanctum in various solvents like acetone, methanol, chloroform, diethyl ether, dimethyl sulfoxide and aqueous extracts. The Minimum Inhibition Concentration (MIC) of UA, OA and BA against pathogenic bacteria was also determined. The methanolic and aqueous extracts of crude leaf powder were found to be most effective against the pathogenic bacterial strains selected for the study. Of the three triterpenoids, UA was most effective followed by OA and BA against most of the pathogens. Furthermore, the concentration of UA and OA in leaf powder of Ocimum sanctum was also determined by HPLC. The total concentration of UA and OA was 0.352% in leaf of Tulsi.