GC-MS Analysis of Metabolites from Endophytic Fungus Colletotrichum gloeosporioides Isolated from Phlogacanthus thyrsiflorus Nees

摘要

Endophytes are those organisms that colonize the living internal tissues of their hosts without causing detectable symptoms. Several fungal endophytes have been isolated from a variety of plant species which have proved as a rich source of secondary metabolites. For the first time Phlogacanthus thyrsiflorus Nees, a well known medicinal plant in Manipur has been explored for its endophytes. In this present work a total of 6 fungal endophytes were isolated from Phlogacanthus thyrsiflorus Nees. Colletotrichum gloeosporioides was the main isolate and were screened for the production of secondary metabolites. The fungus was identified based on the morphological and molecular characterization. Volatile metabolites in the ethyl acetate extract of the Colletotrichum gloeosporioides were determined using Gas chromatography– Mass spectrometry (GC–MS). GCMS analysis revealed the presence of various metabolites. The main constituents were Phenol,2,4-bis (1,1-dimethylethyl), 1-Hexadecene, 1-Hexadecanol, Hexadecanoic acid, octadecanoic acid methyl ester and 1-Nonadecene. The compounds produced by endophytic fungus Colletotrichum gloeosporioides could be an alternative source for human welfare. This study is an important step in investigating the endophytic fungal diversity and to explore the novel bioactive compounds from medicinal plants of Manipur.