THE EFFECT OF DOXYCYCLINE HYDROCHLORIDE ON MMP-2 AND 9 IN EXPERIMENTALLY INDUCED PERIODONTITIS – AN ANIMAL STUDY

摘要

The periodontal tissues undergo constant changes of remodelling when subject to bacterial infection and the resultant host response. The balance between enzymes secreted by the host such as matrix metalloproteinases (MMPs) and their antagonists tissue inhibitor of metalloproteinases (TIMPs) are major contributors to the progression of periodontal destruction. A sub-type of MMPs referred to gelatinases (MMP-2 and 9) are known to be responsible for the pathologic tissue degradation, with MMP-9 playing a potential role in periodontal pocket formation in periodontitis. The use of anti-collagenase host modulatory agents such as doxycycline hydrochloride regulates the extent of resultant tissue destruction and provides an ideal therapeutic approach in the treatment of periodontitis. However, data regarding the effect doxycycline hydrochloride on gelatinases has not been extensively documented. Therefore, the aim of this study was to identify the expressions of MMP-2 and 9 by an immunohistochemical assay in experimentally induced-periodontitis animal models and to evaluate the effects of a prepared doxycycline hydrochloride-impregnated collagen scaffold on their expresssion.