INVESTIGATION ON REGIONAL VARIATION IN TOTAL PHENOLIC, ALKALOID CONTENT AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEUCAS ASPERA

摘要

this study, we investigated and compared the total phenolic, alkaloid content and in-vitro antioxidant activity of Leucas aspera collected from four different regions; Tirupathi (Southern zone), Lam (Krishna river region), Jagityala (Northern Telangana) and Hyderabad (Southern Telangana) of Andhra Pradesh, India. Quantitative regional variation was observed in total phenolic content, and alkaloid content in methanolic extracts of Leucas aspera from above four regions of Andhra Pradesh. Concentration dependent antioxidant activity was observed for all these extracts and also observed regional variation for scavenging of Superoxide, Hydroxyl and DPPH radicals. Among the four regions, Leucas aspera from Jagityala region contains more phenolic content (48.06?à0.4|ìg/100 |ìg), Tirupathi region contains good alkaloid content (58.6?à0.1|ìg/mg) and Hyderabad zone showed better free radical scavenging activity (IC50value for superoxide radical 156.34|ìg, Hydroxyl radical 122.34|ìg and DPPH radical 57.12|ìg respectively