STABILITY INDICATING FORCED DEGRADATION RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF OLMESARTAN MEDOXOMIL

摘要

A simple, precise, accurate, economical and reproducible HPLC method for estimation of Olmesartan in tablet dosage form has been developed. Quantitative HPLC was performed with HITACHI L2130 with D Elite 2000 Software with Isocratic with UV-Visible Detector (L-2400), PUMP (LC-IOAT). C18 Develosil ODS HG-5 RP 150mm x 4.6mm 5μm particle sizecolumn receptor in vascular muscle. Its action is therefore independent of the pathway of angiotensin II synthesis was used in the study.The mobile phase of ACN: phosphate buffer (pH 3.1) was used in this study. The conditions optimized were: flow rate (1.0 ml/minute), wavelength (205nm) and run time was 10 min; column temperature was maintained at 400C. Retention time was found to be 6.31 min. The linearity was found to be in the concentration range of 10-100 mg/ml. The developed method was evaluated in the assay of commercially available tablets OLMESARcontaining Olmesartan. The amount of drug in tablet was found to be 40mg. Results of analysis were validated statistically and by recovery studies. The recovery studies 99.27 % was indicative of the accuracy of proposed method. The precision was calculated as repeatability, inter and intraday variation (%RSD) for the drug. By using the method, stability of the drug has been studied.