The Development of Multiplex PCR Microsatellite Marker Sets for Korean Chicken Breeds

摘要

Background and Objective: Korea’s National Institute of Animal Science (NIAS) has preserved two types of purebred chicken strains and the Hanhyup Company has maintained several purebred chicken stains. This study aimed to facilitate the analyses of genetic diversity , parentage and population structure through the isolation and characterization of novel multiplex PCR microsatellite (MS) marker sets for Korean chicken breeds. Materials and Methods: Genotyping of all 27 markers was performed for 469 purebred chicken samples from 20 Korean breeds. Of these, 16 highly polymorphic MS markers were selected based on the number of alleles, expected heterozygosity (Hsubexp/sub) and polymorphic information content (PIC). The selected markers were classified into two sets of 16 markers each. Results: The expected probability of identity values for the 16 MS markers in random individuals (PI), random half-sib (PIsubhalf-sibs/sub) and random sibs (PIsubsibs/sub) were estimated at 1.79×10sup?/supsup21/sup, 6.76×10sup?/supsup16/sup and 5.70×10sup?/supsup8/sup respectively. Conclusion: Our multiplex PCR marker sets were observed to have favorable applications in the conservation, planning of breeding and traceability systems in the Korean chicken breeds. Additionally, the marker sets will be of considerable use in small- and large-scale population genetic structure analyses.