PHYTOCHEMICAL, FT-IR, AND GC-MS ANALYSIS OF STEM AND LEAF OF TILIACORA ACUMINATA (LAN.) HOOK F & THOMAS (MENISPERMACEAE)

摘要

The present study deals with the phytochemical examination of therapeutic importance of Tiliacora acuminata (Lan) Hook. F & Thomas an important medicinal plant. Qualitative phytochemical analysis of the methanol and ethanol extracts prepared from Tiliacora acuminata stem and leaf revealed the presence of alkaloids, anthraquinones, catechins, coumarins, flavonoids, phenols, quinones, saponins, steroids, sugar, glycosides, tannins and xanthoproteins. The FT-IR spectrum confirmed the presence of alkyl group, methyl group, alcohol group, ethers, esters, carboxylic acid and anhydrides. The bioactive components of the ethanol extract of Tiliacora acuminata stem andleaf were investigated using Perkin-Elmer Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis of ethanol extract ofstem of Tiliacora acuminata revealed the existence of 9,12,15-Octadecatrienoic acid (Z,Z,Z) (85.6%), 9,12,15,Octadecadienoic acid (Z,Z) (7.2%), Asarone (3.5%), Hexadecanoic acid, ethyl ester (1.6%), Methyl-α-d-ribofuranoside (1.4%) and 9,12,15,Octadecadienoic acid, methyl ester (E,E)- (0.8%) and leaf of Tiliacora acuminata revealed the existence of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (14.9%), Squalene (14.5%), Phytol (9.5%), Vitamin E (8.3%) and Heptadecane, 2, 6-dimethyl- (7.9%).