Evaluation of Salmonella Gallinarum Outer Membrane Protein Based Enzyme Linked Immunosorbent Assay for Detecting Antibodies in Vaccinated and Infected Chicken

摘要

Indirect enzyme linked immunosorbent assay was developed to measure specific antibody activity to outer membrane proteins in sera and egg yolk of chickens exposed to Salmonella Gallinarum infection and also following vaccination. The observed end point titers were used to formulate regression equation and the absorbance data obtained at a single serum dilution were converted directly to antibody titer by subtraction method. The assay was carried out at a single serum dilution of 1:320 standardized and the antibody titers of the Salmonella Gallinarum infected flocks were found to be of higher magnitude when compared to vaccinated flocks. The growth-decline trend of the egg yolk antibody levels was found to be similar to that of the serum antibody levels.