Hepatoprotective and Immunostimulatory Effect of Ganoderma , Andrographolide and Turmeric Against Aflatoxicosis in Broiler Chickens

摘要

Objective: The present study aimed to investigate the toxic effects of Aflatoxin (AF) B1 (AFB1) and evaluate the role of Ganoderma lucidum ( GL ) , Andrographolide ( AP ) and Turmeric curcuma ( CM ) in reducing these toxic effects in broiler chickens. Material and methods: A total of 250 (Ross-308) one-day-old broiler chickens were randomly divided into 5 treatment groups (T1-T5) with 50 chicks per group. All groups except for T5 were fed diets that were contaminated with AFs and the groups were treated as follows: T1: Received 0.2% (2 g kg^?1) GL as a feed additive. T2: Received 0.2% (2 g kg^?1) AP as a feed additive. T3: Received 0.2% (2 g kg^?1) CM as a feed additive. T4: Was a positive control (vaccinated but not treated). T5: Was a negative control (not vaccinated or treated). At ages 7, 15 and 25 days, all groups except T5 were vaccinated against Newcastle Disease (ND) ( La Sota ) and at 12 days, they were vaccinated against Infectious Bursal Disease (IBD) (intermediate D78 strain) (in drinking water). Twenty chicks from each group were challenged with a local virulent ND virus (NDV) isolate (ELD₅₀ 10⁵) at 35 days. The AF content was 46.768 ppb in starter diets and 48.661 ppb in the final diet. Results: The GL -fed chicks produced the highest variable antibody titer (Abs), not significant (p hydrogen peroxide (H₂O₂) and malondialdehyde (MDA), significantly different (p lipid peroxidation (LPO) and highly significant (p Conclusion: The study showed the role of GL , AP and CM in reducing the negative effects of AFs by decreasing oxidative stress and immunosuppression in broiler chickens.