Evaluation of insecticide resistance and biochemical mechanisms of Culex pipiens L. in four localities of east and middle mediterranean basin in Turkey

摘要

West Nile virus (WNV) infections in Turkey and East part of Europe have become one of the mostimportant vector-borne diseases. Cx. pipiens L. complex species is a main vector species of the West Nilevirus in these areas. Vector control practice with different classes of insecticides is the most commonlyused alternative for mosquito control. The aim of this survey was to measure three different classes(Organochlorine, Organophosphate and Pyrethroids) of insecticide effectiveness, effects of synergist andpossible biochemical mechanisms of Culex pipiens L. in Turkey.Bioassays results showed that permethrin and deltamethrin toxicity ranged from 50% (Mersin) to 84%(Huzurkent), whereas DDT toxicity was under 50% for all populations, except for Kapikaya (64%). Inaddition, Malathion toxicity ranged from 40% (Mersin) to 74% (Huzurkent). As a result of inhibitorassays, PBO and DEF increased the toxicity related to the insecticide classes. Biochemical assay resultsindicated that the MFO and NSEs (especially p-NPA) played an important role for DDT, malathion andpyrethroid resistance. GST activity assays implied that resistance was multifactorial for all testedpopulations.