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A Novel PCR-Based Approach to Discover miRNA Target Genes

机译:一种基于PCR的新颖方法来发现miRNA靶基因

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摘要

MiRNAs are potent regulators of gene expression, and most miRNAs have from several to several thousands of gene targets. Validating the numerous gene targets of a given miRNA remains challenging despite the existence of various tools and databases that predict candidate gene-miRNA pairs. In the present study, we present a high-throughput but flexible method that applies a PCR-based application to simulate the binding of miRNAs to their gene targets. Using hsa-miR-377 as an illustrative example, our method was able to identify 13 potential targets of hsa-miR-377. Moreover, our results include 2 genes (SOD2 and PPM1A) that have already been verified as targets of hsa-miR-377. Our method may provide an alternative way of identifying the gene targets of miRNAs for future research.
机译:MiRNA是基因表达的有效调节剂,大多数miRNA具有数个到数千个基因靶标。尽管存在各种预测候选基因-miRNA对的工具和数据库,但验证给定miRNA的众多基因靶标仍然具有挑战性。在本研究中,我们提出了一种高通量但灵活的方法,该方法应用基于PCR的应用程序来模拟miRNA与它们的基因靶标的结合。以hsa-miR-377为例,我们的方法能够识别出13个可能的hsa-miR-377靶标。此外,我们的结果包括2个基因(SOD2和PPM1A),这些基因已被验证为hsa-miR-377的靶标。我们的方法可能为鉴定miRNA的基因靶标提供另一种方法,以供将来研究。

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