An Analysis of Polymorphism Lymphotoxin Alpha +252 A>G in South Indian Breast Cancer Patients

摘要

Cytokine tumor necrosis factor contributes to a wide range of functions like inflammation, immunomodulatory and apoptotic activities. Our aim was to investigate the association of plasma levels of lymphotoxin alpha (LTA) with polymorphism rs909253 at +252A>G in primary invasive breast cancer(BC) patients. A total of 146 patients and 150 age matched healthy controls were included in the study. Genotyping was carried out by polymerase chain reaction and restriction fragment length polymorphism. Plasma levels were estimated by The MILLIPLEX? MAP Human Cytokine / Chemokine Panel magnetic bead kits. Data was statistically analysed by R software version 3.3.1. Plasma levels of LTA was significantly increased in cases when compared to controls. However, when levels was analyzed according to polymorphic subtypes there was no significant difference. The polymorphism was in consistent with the Hardy Weinberg(HW) equilibrium, showing X2 values of 2.3 (p= 0.13) and 2.02(p= 0.15) for cases and controls respectively. Odds ratio (OR) indicated that polymorphism was not significantly associated with breast cancer. Plasma levels of LTA were not altered due to polymorphism in patients and controls. Tumors of high- grade and hormone receptor negative cases showed higher frequency of the G allele, indicating that G allele patients may have worse prognosis. This study suggests that LTA +252A>G gene polymorphism is not a prominent risk factor for BC.