Adventitious Bud Induction and Plant Regeneration in Epicotyl Segments of Bakrai ( Citrus reticulata Blancox C. limetta Swing.)

摘要

Experiments were conducted to study the in vitro propagation of Bakrai using epicotyl explants. Explants were excised form in vitro grown seedlings, cultured on a basic MS medium supplemented with various concentrations of benzyladenine (BA) and naphthaleneacetic Acid (NAA). The highest percentage of explants producing normal adventitious shoots (83.3%) was obtained on a medium MS medium containing 2 mg L-1 BA. The maximum number of adventitious buds (20.33) produced in 4 mg L-1 BA, but most of these buds remained quiescent. Addition of 0.5 mg L-1 NAA increased shoot number and shoot length but higher NAA concentrations had an inhibitory effect on shoot regeneration, particularly in 1 mg L-1 BA concentration. The highest number of shoots (5.5) and longest shoots (9.68 mm) were obtained in concentrations of 2 and 1 mg L-1 BA plus 0.5 mg L-1 NAA, respectively. The shoots were rooted on a basal medium with half-strength MS salts and various concentrations of indolebutyric acid (IBA) (0.5-10 mg L-1 ). The highest percentage of rooting (91.67%) and the maximum number (1.71) and length (20.33 mm.) of roots were obtained with 10 mg L-1 IBA. The plantlets were transferred to pots containing an equal mixture (v/v) of sand and loamy soil and successfully acclimatized to natural environment with a survival rate of 80%.