The Association of Beta-catenin Gene Mutations and Human Papillomavirus in Carcinoma of

摘要

Background: Esophageal cancer (EC) is the sixth leading cause of death from cancer. In high-risk regions, squamous cellcarcinoma is the most common type of EC, and its etiology remains poorly understood. It shows uneven geographicaldistribution in its occurrence, reflecting the influence of local environmental conditions, lifestyle and genetic predisposition in thedevelopment of the cancer. Kashmir, in the north of India, has been described as a high-risk area for esophageal squamous cellcarcinoma (ESCC). In the present investigation an attempt was made to study the role of -catenin mutations and humanpapillomavirus in 62 ESCC patients from Kashmir.Methods: The hot spot mutation region of -catenin exon 3 was evaluated in matched tumor and normal tissues using acombination of PCR-SSCP and direct sequencing. We used two different sets of consensus primers viz., GP5+ and GP6+;PGMY09 and PGMY11 in conjunction with reverse line blot assay to screen for human papillomavirus(HPV).Results: None of the tumors showed the presence of commonly reported mutations in -catenin. In view of the fact that HPVhas been linked to pathogenesis of EC, we screened all the tumor and control specimens for the presence of HPV and wedidn?’t detect HPV in any of the matched tumor and control specimens in contrast to the positive controls we used.Conclusion: In conclusion our results suggest that squamous cell carcinoma of esophagus in Kashmir may arise independentof oncogenic -catenin mutations and HPV is unlikely to be an etiologic factor for ESCC in this region.Key words: Esophageal cancer, -catenin gene mutations, human papillomavirusAbbreviations: HPV-human papillomavirus, EC-esophageal cancer, ESCC-esophageal squamous cell carcinoma, PCRpolymerasechain reaction, Rb-retinoblastomaPDFHow to Cite Mir, M., Dar, J. A., Dar, N. A., Syed, A. T., Salam, I., & Lone, G. N. (1). The Association of Beta-catenin Gene Mutations and Human Papillomavirus in Carcinoma of. International Journal of Health Sciences, 1(2). Retrieved from https://ijhs.org.sa/index.php/journal/article/view/81More Citation FormatsACMACSAPAABNTChicagoHarvardIEEEMLATurabianVancouverDownload CitationEndnote/Zotero/Mendeley (RIS)BibTeXIssueVol 1 No 2 (1): Issue 2SectionOriginal Paper Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).Most read articles by the same author(s)Mohammad Muzaffar Mir,Nazir A Dar,Irfana Salam,Zaffar A Shah,Mutations in Epidermal Growth Factor Receptor Gene in Esophageal,International Journal of Health Sciences: Vol 2 No 2 (2): Issue 4Mir M. Muzaffar,Javid A. Dar,Nazir A. Dar,Shafi M. Dar,Irfana Salam,Maqbool M. Lone,Nissar A. Chowdary,Combined Impact of Polymorphism of Folate Metabolism Genes; Glutamate Carboxypeptidase,,International Journal of Health Sciences: Vol 2 No 1 (2): Issue 3Mohammad Muzaffar Mir,Nazir Ahmad Dar,Irfana Salam,Mushtaq Ahmad Malik,Mohamad Maqbool Lone,Ghulam Nabi Yatoo,Aquil Ahmad,Azra Shah,Studies on Association Between Copper Excess, Zinc Deficiency and TP53 Mutations in,International Journal of Health Sciences: Vol 1 No 1 (1): Issue 1Make a SubmissionInformation