Biochemical Identification of Enteroaggregative Escherichia Coli among Infants with Acute

摘要

Background : The EAEC strains have been associated classically with persistent diarrhea which represents a disproportionateshare of diarrheal mortality. EAEC strains have been shown to elicit damage to intestinal mucosa and growth retardation ininfants. Detection of EAEC strains can make a significant contribution to public health in many areas.The use of biofilm assays as a screening method for EAEC from clinical isolates and multiplex PCR as confirmatorymethod may be useful.Methods : We evaluated the usefulness of quantitative micro titer plate method for biofilm production and multiplex PCR toscreen and confirm EAEC from a total of 100 E.coli strains from children below two years of age with acute diarrhea.Results : E.coli strains were isolated from fecal specimens from 680 Diarrheic children who attended an out patient clinic orwho were admitted to Kasturba Hospital and other peripheral hospitals in and around Manipal, Karnataka-South India. E coliisolates from 50 fecal specimens from infants without diarrhea (controls) who attended the same outpatient clinic and whobelonged to the same group as the infants with diarrhea were also examined.Conclusion : Better diagnostic tools are needed to allow for more standardized laboratory testing on a regular basis. Improveddiagnostic tools will help identify epidemiologic patterns of illness and guide treatment recommendations of EAEC illness.PDFHow to Cite Bangar, R., & Mamatha, B. (1). Biochemical Identification of Enteroaggregative Escherichia Coli among Infants with Acute. International Journal of Health Sciences, 1(2). Retrieved from https://ijhs.org.sa/index.php/journal/article/view/92More Citation FormatsACMACSAPAABNTChicagoHarvardIEEEMLATurabianVancouverDownload CitationEndnote/Zotero/Mendeley (RIS)BibTeXIssueVol 1 No 2 (1): Issue 2SectionOriginal Paper Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).Make a SubmissionInformationFor ReadersFor AuthorsFor LibrariansIndexed in PubMed, PubMed Central, EBSCO Host, Emerging Sources Citation Index (Thomoson Reuters), DOAJ, Google Scholar, Microsoft Academic