Genome-Wide Analysis of the ATP-Binding Cassette A (ABCA) Gene Family and their Expression Profiles in High and Low Oil Cultivars of Brassica napus

摘要

ATP-binding cassette (ABC) proteins exist widely in plant cells and ABCA was a subfamily in ABC superfamily. AtABCA9 mediates acyl-CoA/Fatty acids import into Endoplasmic Reticulum (ER) in Arabidopsis. However, the characterization of ABCA genes in Brassica napus L remains unknown. In the present study, 32 members of ABCA gene (BnABCAs) families were identified in the B. napus through homology screening. We performed the analysis of gene structures, chromosomal distribution, phylogenetic relationships and motifs. Meanwhile, we also predicted the subcellular localization and 3D models of partial identified BnABCA members. Furthermore, the expression patterns of most identified BnABCA genes were examined in seeds of different stages of B. napus by RNA-Seq. The result revealed that BnABCA9-1 and BnABCA9-2 were mainly expressed in the seeds during seed oil accumulation stages. Furthermore, the expression levels of BnABCA9-1 and BnABCA9-2 genes were analyzed in seeds of different stages of two high/low oil content cultivars of B. napus by qRT PCR assays. The results imply that the BnABCA9 may be involved in the acyl-CoA export from ER or lipid degradation, which is different from the role of ABCA9 in Arabidopsis.