Effects of Rhizobia and Mycorrhizae Inoculations on the Growth and Nodulation of Chamaecytisus proliferus

摘要

The present study aims to assess the effect of rhizobia and /or mycorrhizae inoculations on the growth and nodulation of Tagasaste "Chamaecytisus proliferus subsp. Palmensis". Three strains of mycorrhizae and twelve rhizobia strains were used. The mycorrhizal inoculation was done at the time of the seedling, mixing the spores of three arbuscular mycorrhizal fungi with a sterile peat. The rhizobial inoculation was performed twice by applying the inoculum that contained a mixture of 12 strains of Rhizobium multiplied before in the Yeast Mannitol Broth medium. The biomass and nodulation were evaluated under different treatments. The essay was realized at the greenhouse the Faculty of Sciences, Moulay Ismail University. The Inoculation with these root symbionts, separate or combined, increased the biomass accumulation of the Tagasaste treated compared to the absolute control. However, the best response result was that of the simple rhizobial inoculation which showed a very good growth as well as an important root infection assisted by the number and weight nodule compared to the single mycorrhizal inoculation and to the dual inoculation. Finally, the symbiosis with rhizobia and mycorrhizae is an important biological technology to improve the sustainable production of leguminous plant in different agro-ecological regions.