IN VITRO PROPAGATION OF ALTERNANTHERA VERSICOLOR (LINN.) - A MEDICINAL HERB

摘要

A complete protocol for micropropagation of Alternanthera versicolor using leaf and internodal explants was developed. An explants from A. versicolor established on Murashige and Skoog medium were treated with various concentrations and combinations of auxins and cytokinins to determine the best method for callus formation, shoot regeneration and root formation. MS medium supplemented with 1 mg/l BAP and 1 mg/l IAA was determined to be the most suitable for callus induction. After 2 weeks, the callus was then transferred to shooting media which consisted of half strength MS basal medium, 1 mg/l IAA and 1 mg/ l BAP. After 4 weeks, structures were observed with their basal ends embedded on the callus. These structures turned into purple colored shoot buds. MS medium supplemented with 1 mg/l IAA and 1 mg/l BAP showed maximum shoot multiplication. For rooting, the optimal medium was half strength MS medium supplemented with 1 mg/l IAA. Rooted plantlets were then transferred to sand bag vessels which provided the high humidity environment. The hardened plants were then successfully established in the soil medium and can function in the natural environment.