Comparative Study of in-vitro Behavior of Tetracalcium Phosphate-based Cement: Ringer’s Solution versus Human Blood Plasma

摘要

Apatite formation ability of an implant in a supersaturated solution has been widely used to investigate its bioactivity. However, the method may provide incomplete information, because it is determined only by solution supersaturation, irrespective of biological factors. In this study, apatite-forming ability of calcium phosphate cement (CPC) was evaluated in two different media; human blood plasma and Ringer’s solution. The CPC samples were stored in human blood plasma and Ringer’s solution for different intervals and change in phase composition, microstructure and compressive strength were investigated. The reactants could be transferred to apatite phase in both Ringer’s solution and human blood plasma but the crystallinity and size of the apatite crystals was higher for the former. Furthermore, the compressive strength of sample fourteen days after soaking in Ringer’s solution was 1.6-fold of that in human blood plasma. Overall, the in vitro tests in human blood plasma which is more similar to real conditions can produce some results different from Ringer’s solution.