Hydrodynamic Voltammograms Profiling of Metallothionein Fragment

摘要

Metallothionein is intracellular metal binding protein. It can bind essential zinc ions and also toxicmetals like cadmium. In cancer research the problem of resistance of cancer cells against anticancerdrugs was described. In this study we optimized an electrochemical method for exploring thedifferences in electrochemical signals in dependence on amino acid sequence in metallothionein. Forthis purpose we selected the number of fragments based on bioinformatic processing, synthesized themand made high throughput electrochemical analysis. Designed electrochemical analysis was based onautomated flow injection analysis with amperometric detector using Coulochem III. Detection wascarried out by glassy carbon electrode. We measured hydrodynamic voltammograms (HDVs) for 23selected metallothionein fragments within the potential range from 100 to 1200 mV. We estimated themaxima of oxidation by processing of cumulative HDV. The oxidation maxima were dependent onamino acid composition, whereas fragments from human isoforms and some saltwater fishesspecieshad the highest maxima (900 mV). On the opposite side fragments of other mammals like horse,mouse and bull had the lowest potential maxima (700 mV). This should be related with the bindingcapacity for metal ions.