A Novel Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized on Poly(aniline-co-o-aminobenzoic acid) Modified Glassy Carbon Electrode Coated with Chitosan Film

摘要

In this work, a novel amperometric biosensor for hydrogen peroxide (H2O2) determination wassuccessfully prepared by immobilizing horseradish peroxidase (HRP) on poly(aniline-co-o- aminobenzoic acid) or p(Ani-co-o-Aba) and then covered with chitosan (CS) film. The immobilizedHRP displayed an excellent electrocatalytic activity to the reduction of hydrogen peroxide. The effectsof experimental variables such as the o-Aba mol ratios in p(Ani-co-o-Aba) synthesis, HRP and CSconcentrations, pHs of supporting electrolyte solution and applied potentials for the working electrodewere investigated for the optimized conditions. This novel biosensor exhibits a fast response towardH2O2 with a linear range from 10 to 1,000 and a detection limit of 1.8 based on the signal-to- noise ratio (S/N = 3). The developed biosensor shows satisfactory tolerance with other potentialinterferences such as dopamine (DA), ascorbic acid (AA), glucose (Glu) and uric acid (UA), and alsoshows a good stability for weeks.