Determination of Non-Steroidal Anti-Inflammatory Drug (NSAID) Azathioprine in Human Blood Serum and Tablet Samples Using Multi-Walled Carbon Nanotubes (MWCNTs) Decorated Manganese Oxide Microcubes Composite Film Modified Electrode

摘要

The development of sensor for azathioprine (ATP) determination is extremely important as its excess level in blood imposes carcinogenic threats. A sensitive, selective, reproducible and durable azathioprine sensor was demonstrated using a nanocomposite composed of Mn3O4 micro cubes and multi-walled carbon nanotubes (MWCNTs/Mn3O4 MCs). The composite was prepared through a facile hydrothermal method and its morphology, elemental, impedance and electrochemical properties were studied. The MWCNTs/Mn3O4 MCs was modified on the screen-printed electrode, and the resulting modified electrode displayed excellent electrocatalytic ability towards ATP and the reduction peak current was related to the concentration of ATP. The working range was 0.045-2530 μM and the detection limit was 4.3 nM (± 2.53). The method was successfully applied to the determination of ATP in spiked samples of human blood serum and tablets.