Biosynthesis of Gold Nanoparticles Using Pleurotus ostreatus extract with Their Electrochemical Activity of Detection of Carbendazim in vegetable

摘要

Biosynthesis has attracted numerous attentions recently in the field of nanomaterial synthesis owing to its non-toxicity and environmental protection. Herein, AuNPs were successfully prepared by biosynthesis with Pleurotus ostreatus produced laccase as reducing agent. The formation of metallic Au was confirmed by both UV-vis spectroscopy, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). As shown form the results of scanning electron microscopy, the mean size of the biosynthesized AuNPs were 47 nm. The biosynthesized AuNPs were then applied for the modification of screen printed electrode. The electrochemical sensor constructed with AuNPs/SPE electrode demonstrated remarkable performance towards the determination of carbendazim in vegetable.