Electrocatalytic Oxidation of NADH on Graphene Oxide and Reduced Graphene Oxide Modified Screen-Printed Electrode

摘要

A facile method, Graphene Oxide (GO) modified electrochemically pre-anodized screen-printedcarbon electrodes (SPCEs), was developed for the determination of nicotinamide adenine dinucleotide(NADH) in neutral aqueous solution. Compared with the bare SPCEs and the reduced Graphene Oxide(rGO) modified SPCEs (rGO/SPCEs), the GO/SPCEs exhibit greatly enhanced electrocatalyticoxidation toward NADH. The scanning electron microscopy (SEM) and X-ray photoelectronspectroscopy (XPS) provide the substantial evidence for the formation of edge-plane sites on thesurface of SPCEs, rGO/SPCEs and GO/SPCEs. The GO/SPCEs show the excellent electrochemicalcharacter for the determination of NADH in the range from 0.8 to 500 M with a detection limit of+ 0.10 M. The formation of oxidation product NAD was confirmed by in-situ UV-Visspectroelectrochemistry. It shows a great promise for the design of the disposable amperometricbiosensor for NADH.