Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase- glutaraldehyde-cysteine layers on gold electrode

摘要

Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) ontogold electrode is constructed and already electrochemically tested. Now, the electrochemical behaviorof biosensor in human serum is further investigated and supported by morphological characterizationof layers for the first time. The morphology and microstructure of layers was examined by Fouriertransformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). Theelectrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that therewere some bare regions which remain catalytically active is supported by AFM and OM results. Theconstruction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIRstudy.