Development and Validation of Electrochemical Method for Quantification of Palbociclib (Anticancer Agent) in Biological Matrices Using Square Wave- Adsorptive Stripping Voltammetry

摘要

The electrochemical reduction behavior of palbociclib (PLB) was studied using square wavevoltammetry, differential pulse polarography and cyclic voltammetry in Britton-Robinson (B-R) bufferof pH 7.0. The mercury electrode was used to accumulate PLB into its surface to give a well-definedreduction wave at -1.05 V potential in the presence of Ag/AgCl reference electrode and Pt auxiliaryelectrode. There were some analytical parameters which studied to obtain the best reduction signal,such as buffer solutions (types and strength), pH values, scans and stirring rates. Britton-Robinsonbuffer of pH 7.0, 50 s accumulation time, 0.0 V accumulation potential, 30 Hz frequency, 300 mV s -1scan rate, 50 mV amplitude, 0.6 mm 2 drop area, and 3000 rpm were recorded high sensitivity for thePLB determination, so they were chosen as optimum parameters for the next work. The repeatability,stability, recovery, calibration curve and detection limit were validated to evaluate the analyticalperformance of the developed method. Repeatability and stability of 5 х10 -7 mol L -1 of PLB werereported 0.0282% relative standard deviation (RSD%) for ten cathodic measurements, and a goodstability for 120 min, respectively. Calibration curve was studied over the range 1х10 -7 - 1 х 10 -6 molL -1 for PLB to be obtained a linear relationship with a 0.992 correlation coefficient (r 2 ) for sexmeasurements (n = 6). Lower detection limit (LOD) was calculated to be 8.8 х 10 -11 mol L -1 (0.039ppb), while lower quantification limit (LOQ) was become 2.9 х 10 -10 mol L -1 (0.131ppb). The squarewave voltammetry method was applied for qantification of PLB at the human plasma and urinesamples.