Effect of dexamethasone on unfolded protein response genes (MTJ1, Grp78, Grp94, CHOP, HMOX-1) in HEp2 cell line

摘要

The endoplasmic reticulum (ER) is related to the various signal routes that are activated in unfolded protein response (UPR). The iGrp78, Grp94, CHOP, MTJ1/i and iHMOX1/i genes expressions demonstrate UPR activity. In this study, we investigated the UPR gene expressions in larynx epidermoid carcinoma (HEp2) to which dexamethasone (dex) was applied. HEp2 cells were administered for 48 h with different combinations using 0.1 μM and 1 μM dex, 1 mM phenyl butyric acid (PBA) and 100 ng/ml lipopolysaccharide (LPS). The iGrp78, Grp94, CHOP, MTJ1/i and iHMOX1/i genes expression was determined using quantitative RT-PCR. The ispan style="mso-ansi-language:EN-US" lang="EN-US"Grp78, MTJ1/span/ispan style="mso-ansi-language:EN-US" lang="EN-US" and iHMOX1/i gene expression increased with the administration of 1 μM dex. iCHOP/i expression, on the other hand, decreased with 0.1 μM dex. When dex was combined with LPS, nearly all gene expressions decreased. The increase in iGrp78, Grp94, HMOX1/i and iMTJ1/i gene expression was greater in groups in which dex was administered in combination with PBA than in groups in which dex was administered alone. span style="mso-ansi-language: EN-US" lang="EN-US"Dex in low dose (0.1 μM) caused a decrease in iCHOP/i expression in HEp2 cells and an increase in iGrp78/i expression, in particular. The changes in UPR genes expressions may lead to the extended survival of the cells. /span/span" xml:lang="en_US