Effect of Total flavonoid extract of Tanacetum parthenium L. (Feverfew) pollen grains on immune system responses in Balb/C mice

摘要

Tanacetum parthenium L. has been used in traditional medicine especially for reducing fever and as an insectrepellant. This plant has some pharmacological properties, such as anticancer, anti- inflammatory, and it’s alsocardiotonic, antispasmodic and has emmenagogue properties and it can also be used as an enema for worms. Inthis study total flavonoid extract of T.parthenium pollen grains was evaluated for Immunomodulatory activity inmale Balb/C mice by two methods: 1. Delayed type hypersensitivity (DTH) test, 2. Lymphocyte proliferationassay by the method MTT ((3-4, 5-dimethyl tetrazolyl-2) 2, 5 diphenyl) tetrazolyum bromide). Forty five Balb/Cmice were classified in nine groups and treated with eight doses (1, 5, 10, 20, 30, 50, 70 and 100 mg/Kg) of totalflavonoid extract. DTH response was significantly (P<0.01) increased at doses of 20, 30, 50 and 70 mg/Kg. Thehighest DTH response was observed at doses of 50 and 70 mg/Kg of the extract. A significant increase (P<0.01)in lymphocyte immune response was observed at all doses of the extract but the best response was found at thedose of 30 mg/Kg of extract. The results obtained in this study indicated that total flavonoid extract ofT.parthenium pollen grains has significant immunomodulatory activity.