Saccharomyces cerevisiae as a model for the evaluation of dihydropyridine calcium antagonist effects

摘要

In this work, we are interested to using Saccharomyces cerevisiae as eukaryotic model and an alternative one toanimal testing for bringing out the effect of nifedipine, a dihydropiridine L-type calcium channel blocker. Thus, aculture of Saccharomyces cerevisiae was treated with different concentrations of this xenobiotic (0.01, 0.05, 0.5,1 mM) during 3 hours. After that we measured the levels of some stress biomarkers (GSH, GST and peroxidasesactivities). The results obtained show a significant/high significant increase in all studied biomarkers followingtreatment with a dose-response manner. These data highlight an oxidative stress induced by the presence of thecalcium antagonist, and expressed by a stimulation of the oxidative enzymes activities