Evaluation of Analgesic and Anti-Inflammatory Activities of hydro-alcoholic leaf extract of Hypericum revolutum in Mice

摘要

Background: Despite the progress that has been made in the past for the development of pain and inflammation therapy, there is a need for effective and potent medicinal agent with lower side effects. The aim of this study was to evaluate the analgesic and anti-inflammatory activities of 80% methanol leaf extract of Hypericum revolutum in mice.Method: After extraction of the crude using 80% methanol, Hypericum revolutum extract was evaluated for analgesic and anti-inflammatory activity in hot plate test, acetic-acid induced writhing and carrageenan induced paw edema models. Extract was evaluated at 100, 200 and 400 mg/kg doses. The reference control groups were treated with morphine 5mg/kg or indomethacin 10mg/kg while vehicle, distilled water (10 ml/kg), treated animals were grouped as negative controls.Result: In the hot plate method, all doses of the extract and the standard drug morphine significantly prolonged the latency time in the hot plate method (p0.05 or p0.01 or p0.001) when compared to the negative control throughout the observation time. Greater increase in latency was observed at the 120 minute and greater on the other time intervals with 200mg/kg of the extract with a significant value (p0.001). Similarly, all test doses of the extract significantly (p0.05) reduced the acetic acid induced writhing in mice. The extract of HR produced a significant analgesic activity with 62.4, 47.1 and 55.3% inhibition of number of writhing at 100, 200 and 400 mg/kg dose levels, respectively although the inhibition was greater for the standard.  Moreover, for carrageenan induced paw edema test treatment with the extract (100, 200, and 400mg/kg) and the standard have shown significance (p0.01 or p0.001) suppression of edema at all-time points as compared to controls.Conclusion: The 80% hydro-methanolic extract of H. revolutum exhibited analgesic and anti-inflammatory effects in mice models.