Chemical composition and antifungal activity of essential oils of Haplophyllum tuberculatum (Forssk.) A. Juss. from South Algerian

摘要

Utilization of natural products as control agents reveals a biological preventive approach to reduce significantly the use of pesticides and in particular fungicides.In the present study we determined chemical composition of an essential oil extracted from Haplophyllum tuberculatum (Forssk.) A. Juss. Three fungi tomato pathogens are tested to examine antifungal activity of the extracted oil. The essential oil was extracted by hydrodistillation and the chemical composition was determined by gas chromatography coupled with flame ionization detector (GC-FID). Various concentrations (2.5, 5, 10, 20 and 30 mg / ml) of the essential oil were used to investigate in vitro the antifungal activity on a solid agar medium against FORL, B. cinerea and A. solani. The percent of the fungal inhibition was calculated and the ranges of MICs were determined for each fungal isolate tested. The results indicate that the yield of essential oil obtained by hydrodistillation is 0.101%. The major components identified in the essential oil of H. tuberculatum were Piperitone (13.35%), Germacren-B (12.30%) and Beta-Phellandren (5.05%). In addition, the essential oil at 10mg / ml, 20mg / ml and 30mg/ml inhibited completely the growth of B.cinerea, A.solani and 90% of FORL. The two first concentrations were found to be the MIC of A.solani and B.cinerea respectively. The present study shows clearly the antifungal power of the essential oil of H. tuberculatum.