Relation between the changes in IL-6 and glucose in response to exercise test and investigation of physicochemical properties of IL-6 and its ligand binding site

摘要

Obesity is known to be associated with systemic inflammation. In this study, we investigated serum Interleukin 6 and glucose concentration (IL-6) in response to a stepwise cycling test in obese men. For this purpose, serum IL-6 and glucose concentration were measured in eighteen none-trained adult obese men. Serum IL-6 increased significantly and glucose concentration decreased by exercise test when compared to pre test. Changes in IL-6 were negatively correlated with change in glucose concentration. In present study, also, the structure of protein IL-6 was selected from the protein data bank (PDB code 1ALU). We choose Monte Carlo and AMBER force fields for molecular mechanics calculations. Temperatures of calculation were kept 288-315 K. Moreover, additional parameters were calculated using the QSAR PropertiesModule of HyperChem 8.6 software.We measured partial derivative of each subunitin constant and variable volume and temperature. This survey results will help us find the best thermodynamics properties for IL6 and finding the best active site for activity in different state of temperature