In this study five wild medicinal plants viz. Caltha alba , Matricaria chamomilla, Ocimum basilicum, Phyllanthus emblica and Valeriana wallichii were screened to examine their proximate composition, mineral profile, phytochemical constituents, antioxidant and antimicrobial activity. The results of proximate analysis showed that promising moisture (10.20%), fat (3.63%) and protein (11.50%) was recorded in P. emblica while ash (8.87%) and fiber (14.90%) was noted in M. chamomilla and C. alba . However, least amount of moisture (6.92%), fiber (6.10%) and protein (7.42%) was examined in V. wallichii whereas ash (5.07%) and fat (1.56%) was found in C. alba and M. chamomilla . Among all the plant species maximum NFE was observed for V. wallichii (70.19) followed by M. chamomilla (60.23%) while minimum value was reported in P. emblica (57.99%). The results of mineral profile showed that all the selected plants possess notable amount of C, O, Mg, Si, P, K and Al however, S and Cl was not detected in V. wallichii while Ca was not found in O. basilicum . Phytochemical screening revealed that among all the plant species promising total phenolics (95.25 mg GAE gSUP-1/SUP), total flavonoids (61.33 mg QE gSUP-1/SUP) and antioxidant activity (84.70%) was recorded in P. emblica, V. wallichii and M. chamomilla whereas lowest values was investigated in O. basilicum (10.25 mg GAE gSUP-1/SUP), C. alba (8.0 mg QE gSUP-1/SUP) and V. wallichii (65.40%). The antimicrobial potential of crude methanol extract of the examined plant species was tested against opportunistic bacterial and fungal strains by using well diffusion assay. Among all the plant species M. chamomilla exhibits promising inhibitory potential against S. aureus ( 74.07%) followed by A. tumfaciens (69.23%), C. michiganensis (59.25%) and B. subtilis (53.57%). Likewise, V. wallichi have considerable activity against E. coli (56.0%) and X. compestris (46.15%). In case of fungicidal activity notable inhibitory zone was recorded for V. wallichii and P. emblica against Pacelomyces spp., (77.27% and 57.0%) followed by V. wallichii against A. niger (68.42%), however the selected plant also exhibited moderate to good and no activity against the tested fungal strains. It was concluded from the present results that antioxidant and antimicrobial potential of the plant extracts might be due to the presence of total phenols and total flavonoids content.
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机译：在这项研究中，有五种野生药用植物。筛选了Caltha alba，Matricaria chamomilla，Ocimum basilicum，余甘子余甘子（Phyllanthus emblica）和Valeriana wallichii以检查它们的近邻成分，矿物质概况，植物化学成分，抗氧化剂和抗菌活性。最近的分析结果表明，在余甘假单胞菌中记录到有希望的水分（10.20％），脂肪（3.63％）和蛋白质（11.50％），而在沙棘和沙门氏菌中记录到灰分（8.87％）和纤维（14.90％）。 C. alba。然而，在沃氏弧菌中检测到最少的水分（6.92％），纤维（6.10％）和蛋白质（7.42％），而在白毛隐孢子虫和沙门氏菌中检测到灰分（5.07％）和脂肪（1.56％）。 。在所有植物物种中，观察到沃氏弧菌的最大NFE（70.19），其次是沙门氏菌（60.23％），而在余甘假单胞菌中报告的最小值最小（57.99％）。矿物剖面结果表明，所有选择的植物均具有显着量的C，O，Mg，Si，P，K和Al，但在V. wallichii中未检出S和Cl，而在O.basicumum中未发现Ca。植物化学筛选显示，在所有希望有酚类总含量（95.25 mg GAE g -1 ），总黄酮类成分（61.33 mg QE g -1 ）和抗氧化活性的植物物种中（84.70） ％记录在甘蓝假单胞菌，沃利奇菌和沙门氏菌中，而最低值则在罗勒叶青霉菌（10.25 mg GAE g -1 ），白念珠菌（8.0 mg QE g < SUP> -1 ）和壁球菌（65.40％）。通过使用孔扩散测定法测试了所检查植物物种的粗甲醇提取物的抗微生物潜力，以对抗机会细菌和真菌菌株。在所有植物物种中，chamomilla表现出对金黄色葡萄球菌（74.07％）的有希望的抑制潜力，其次是根癌农杆菌（69.23％），密歇根梭菌（59.25％）和枯草芽孢杆菌（53.57％）。同样地，沃利弧菌对大肠杆菌（56.0％）和孔雀志贺菌（46.15％）具有相当大的活性。在杀真菌活性的情况下，记录到的沃氏弧菌和余甘假单胞菌对Pacelomyces spp。（77.27％和57.0％）的抑制区域明显，其次是沃氏弧菌对黑曲霉（68.42％），但是所选植物也表现出了抑制作用。中等至良好，对被测真菌菌株无活性。从目前的结果可以得出结论，植物提取物的抗氧化和抗菌潜力可能是由于总酚和总黄酮含量的存在。
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