Effect of Abiotic Elicitors on Tissue Culture of ALOE vera

摘要

Aloe vera L. belong to the Aloaceae family and actually is a commercial and medicinal important plantbecause of the secondary metabolites. Anthraquinones are well- known and widely used in medicine and foodchemistry. It has dermatological usage with antimalarial, antifungal and antibacterial properties. Aloin is themost important secondary metabolite in Aloe vera, not only due to the medicinal and cosmetic properties butalso due to their physiological role. Plant cell cultures have been used industrially for the synthesis of secondarymetabolites. In this study we have investigated the effects of different abiotic elicitors including Nano-Ag, Nano-Tio2, NH4NO3 and sucrose on cell suspension culture of Aloe vera. The induced callus by elicitors werecollected in five period of time and have been analyzed by HPLC (High-performance liquidchromatography). The results showed that the highest amount of aloin obtained with NH4NO3 at 48hours aftertreatment, and it was 127% higher than control. Aloin content was enhanced with Nano elicitors at 48 hours aftertreatment and decreased gradually after that. Moreover, aloin was increased gradually up to168 hours aftersucrose treatment. These experiments showed that in vitro culture of Aloe vera can be induced to produce moresecondary metabolites.