Designing Microbial Sensors based on Synthesis, Characterization and Sensing Application of Nanocomposite Prepared using Nanoparticles and Conducting Polymer

摘要

Many types of microbial sensors have been developed asanalytical tools. Such a microbial sensor consists of a transducer anda microbe as the sensing element. Originally the microbial sensorslacked in specificity as compared to enzyme sensors orimmunosensors, which are highly specific for the substrates ofinterest, but with advancement of research the specificity of themicrobial sensor has been improved significantly by geneticmodifications of the microbe used as the sensing element. Microbialsensors have the added advantages of a tolerance to the measuringconditions, a long lifetime, and cost performance. Since theirdiscovery in the mid-1970s, research on conducting polymers (CPs)has become an ever-growing research area in polymer chemistry.The redox behaviour and an unusual combination of the properties ofmetals and plastics make conducting polymers a preferred class ofmaterials for composite formation. We herein report the synthesis ofnanoparticles decorated- graphene oxide sheets by a one-potsolution-based method. Further, polymer was introduced into thisnanoparticle decorated graghene oxide sheets to prepare ananocomposite by electropolymerization using potentiodynamicmode on indium tin oxide electrode. The synthesized nanocompositewas characterized by different techniques such as transmissionelectron microscopy, scanning electron microscopy, cyclicvoltammetry etc. The surface morphology studies show that afterintroduction of polymers the morphology of prepared materialchanges. The prepared nancomposite electrode was further used asgenosensor.