Genetic variation among the wild and hatchery raised populations of Labeo rohita (Hamilton, 1822) revealed by RAPD markers

摘要

The studies on genetic diversity of Labeo rohita by using molecular markers were carried out to investigate thegenetic structure by RAPAD marker and the levels of polymorphism and similarity amongst the different groupsof five populations of wild and farmed types. The samples were collected from different five locations asrepresentatives of wild and hatchery raised populations. RAPAD data for Jaccard’s coefficient by following theUnweighted Pair Group Method with Arithmetic Mean (UPGMA) for Hierarchical Clustering of the similargroups on the basis of similarity amongst the genotypes and the dendrogram generated divided the randomlyselected individuals of the five populations into three classes/clusters. The variance decomposition for theoptimal classification values remained as, 52.11% for within class variation while 47.89% for the between classdifferences. The Principal Component Analysis (PCA) for grouping of the different genotypes from the differentenvironmental conditions was done by Spearman Varimax rotation method for bi-plot generation of the cooccurrenceof the same genotypes with similar genetic properties and specificity of different primers indicatedclearly that the increase in the number of factors or components was correlated with the decrease in eigenvalues.The Kaiser Criterion based upon the eigenvalues greater than one, first two main factors accounted for 58.177%of cumulative variability.