PROCESS OPTIMIZATION OF L-GLUTAMINASE PRODUCTION BY Trichoderma koningii UNDER SOLID STATE FERMENTATION (SSF)

摘要

Solid state fermentation (SSF) was carried out for the production of L-glutaminase by the fungal strain Trichoderma koningii using sesamum oil cake as the solidsubstrate. L-glutaminase has received significant attention in recent years owing to its potentialapplications in medicine as an anticancer agent, as an efficient anti-retroviral agent and as abiosensor. In food industries it is used as a flavor and aroma enhancing agent. The overallmaximum yield of L-glutaminase (19.41 U/gds) was achieved with the optimized processparameters of initial moisture content 65%, initial pH of the medium 7.0, supplemented with D-maltose (1.0% w/v) and malt extract (1.0% w/v), inoculated with 2ml of 6 day old fungal cultureand incubated at 33°C for 5 days.Both physico-chemical and nutritional parameters had played asignificant role in the production of the enzyme, L-glutaminase. The enzyme production wasfound to be growth associated with the growth of the fungal culture